Conventional multiplex polymerase chain reaction ( PCR ) versus real - time PCR for species - specific meat
نویسندگان
چکیده
Simple and reliable conventional multiplex-polymerase chain reaction (multiplex-PCR) as well as real-time PCR approaches for the partial-length cytochrome b or cyt b gene of mitochondrial DNA (mtDNA) were compared to identify seven animal species for the authenticity of low non Halal (pig, dog, cat and donkey) and high value Halal (cattle, sheep and goat) meats. Either analysis indicated the successful detection of as little as 0.05 pg (5%) adulteration in cattle meat. As compared to real-time PCR, the approach of conventional multiplex-PCR can also be applied to detect authentication with equal efficiency to fresh, cooked or putrefied mixed samples of cattle meat. [S. Edris, M.H.Z. Mutwakil, O.A. Abuzinadah, H.E. Mohammed, A. Ramadan, N.O. Gadalla, A.M. Shokry, S.M. Hassan, R.M. Shoaib, F.M. Domyati and A. Bahieldin. Conventional multiplex polymerase chain reaction (PCR) versus real-time PCR for species-specific meat authentication] Life Sci J 2012;9(4):58315837]. (ISSN: 1097-8135). http://www.lifesciencesite.com.870
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